Novel regulators and interactions within the regulatory networks controlling the autotrophy -heterotrophy switch in cyanobacterial carbon metabolism
Several lines of evidence indicate that important regulators, activities and signaling chains involved in the control of cyanobacterial primary metabolism have remained unidentified so far. Within the scope of the SCyCode research network, we focus on the identification of novel regulators and regulatory elements. We utilize global approaches such as density gradient ultracentrifugation for the fractionation of protein, protein-metabolite and protein-RNA complexes, followed by proteomic, metabolomic and transcriptomic analyses and extensive correlation profiling to identify regulatory interactions that have remained unknown thus far (Riediger et al., 2021). A database to access the pre-analyzed Grad-seq data can be found here: https://sunshine.biologie.uni-freiburg.de/GradSeqExplorer/
In more detailed experiments we address the roles of specific sRNA regulators (Zhan et al., 2021) and of promising protein candidates identified by proteogenomic approaches (Krauspe et al., 2021) and elucidate the regulatory networks controlled by particular transcription factors.
(Krauspe et al., 2021): https://www.pnas.org/content/118/5/e2012277118.short
(Riediger et al., 2021): https://academic.oup.com/plcell/advance-article/doi/10.1093/plcell/koaa017/6017179
(Zhan et al., 2021): https://academic.oup.com/plcell/advance-article/doi/10.1093/plcell/koaa030/6033669?login=true
Prof. Wolfgang Hess
University of Freiburg
Institute of Biology III
Genetics and Experimental Bioinformatics
Schänzlestr. 1, 79104 Freiburg, Germany
Alexander Kraus M.Sc.
Dr. Matthias Riediger